E. coli expression
For ease of manipulation, cost effectiveness and speed of production. We have designed a series of expression vectors (pINX) that offer ample choices of tagging for solubility enhancement, purification and downstream processing with tag cleavage possibility.
Once subcloned, expression screening is streamlined in carefully selected commercial and in-house modified E. coli strains for optimal expression of soluble protein. Scale-up is achieved in shake flasks up to 10L scale or fermenters. We typically aim for 20-30 mg of >95% pure protein for the structural work but can deliver hundreds of milligrams if your assay requires it.